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Literature summary extracted from

  • Liu, J.; Liu, J.; Straby, K.B.
    Point and deletion mutations eliminate one or both methyl group transfers catalysed by the yeast TRM1 encoded tRNA (m22G26)dimethyltransferase (1998), Nucleic Acids Res., 26, 5102-5108.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.216 expressed in Escherichia coli and the His-tagged Trm1 protein Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.216 E47A mutant enzyme shows 6% of wild-type activity Saccharomyces cerevisiae
2.1.1.216 G48A mutant enzyme shows 16% of wild-type activity Saccharomyces cerevisiae
2.1.1.216 additional information comparatively short N- or C-terminal deletions from the 570 amino acid long Trm1 polypeptide decreased or eliminated the enzyme activity. The first 27 N-terminal amino acids in rTrmp1 are not needed for G26 modification activity in vitro or in vivo. DELTAC5aa causes the in vitro modification activity to drastically decrease to 27% of the wild-type rate. Additional deletions up to DELTA30aa further lower the activity to about 7% of the wild-type value. In DELTA10aa an increase of the N2-methylguanine26 relative to N2-dimethylguanine26 tRNA is observed, but with further C-terminal deletions the monomethylation activity is also severely lowered Saccharomyces cerevisiae
2.1.1.216 S467L complete loss of enzyme activity in vitro against trm1 yeast tRNA Saccharomyces cerevisiae
2.1.1.216 S570A fully active mutant enzyme Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.216 64050
-
 calculated from sequence Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.216 Saccharomyces cerevisiae P15565
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.216
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.1.1.216 4°C, the enzyme precipitates if stored for several days Saccharomyces cerevisiae
2.1.1.216 frozen in small portions, enzyme maintains activity for at least several months Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNA wild-type enzyme produces 94% N2-dimethylguanine26 tRNA and 6% N2-methylguanine26 in tRNA Saccharomyces cerevisiae 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.216 Trm1
-
 Saccharomyces cerevisiae
2.1.1.216 tRNA (m22G26)dimethyltransferase
-
 Saccharomyces cerevisiae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.216 Saccharomyces cerevisiae calculated from sequence
-
 9.2